Regardless of the year, the 2UZ-FE ECU pins are generally divided into four functional groups: 1. Power and Ground (The Essentials) Constant 12V power from the EFI relay.
Because there are over a dozen different plug configurations for the 2UZ-FE, you should verify your ECU part number (e.g., ) against a factory service manual. 2uzfe ecu pinout
Most 2UZ-FE ECUs from 2000 onwards are "locked" to a specific transponder key. If you don't have the original key and the amplifier ring, the engine will start for 2 seconds and die. You may need an Immobilizer Bypass or a "virginized" ECU. Regardless of the year, the 2UZ-FE ECU pins
Constant 12V from the battery (keeps the ECU memory alive for fault codes). Most 2UZ-FE ECUs from 2000 onwards are "locked"
These introduced Drive-by-Wire (ETCS-i) . The pinouts changed to accommodate the electronic throttle motor and pedal position sensor.